3000 Series Nanosphere* Size Standards

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3000 Series Nanosphere* Size Standards

Thermo Scientific* 3000 Series Nanosphere Size Standards are excellent for use with any application that requires a NIST traceable size standard with a very narrow standard deviation.
Nanosphere Size Standards are ideal for the calibration of electron and atomic force microscopes. They are also used in laser light scattering studies and colloidal systems research. The 20 to 900nm range of spheres is convenient for checking the sizes of bacteria, viruses, ribosomes and sub-cellular components.

Thermo Scientific 3000 Series Nanosphere Size Standards are highly uniform polystyrene spheres calibrated within billionths of a meter (nanometers) with NIST traceable methodology. One nanometer is 0.001 micrometer (µm) or 10 Angstroms.

  • Available as uniform polymer spheres in a range of discrete sizes from 20 to 900 nanometers (nm)
  • Spherical diameters calibrated with linear dimensions transferred from NIST by methods developed at Thermo Fisher Scientific
  • Spheres are used instead of irregularly shaped particles to minimize response of analytical systems to shape effects
  • Standards packaged as aqueous suspensions in 15mL dropper-tipped bottles
  • Concentrations optimized for ease of dispersion and colloidal stability
  • Spheres have a density of 1.05 g/cm3 and an index of refraction of 1.59 @ 589nm (25°C)
  • Packages are lot-numbered for convenient technical service and support after the sale

Includes: Certificate of Calibration and Traceability to NIST, including description of the calibration method and its uncertainty, table of chemical and physical properties, and Material Safety Data Sheet, with handling and disposal instructions.
Thermo Fisher Scientific has its own engineering, production and metrology facilities for better quality control and response to customer needs. In addition to this line of polymer size standards, we also offer calibration standards in silica and glass materials. Ask our technical service department for information about specific applications or other product needs not listed here.


  • Composition : Polystyrene
  • Particle Density : 1.05 g/cm3
  • Refractive Index : 1.59 @ 589nm (25°C)
  • Size : 15mL
  • Stability : Product stable for a minimum of 24 months
  • Additive : Contains trace amount of surfactants
  • Includes : Material Safety Data Sheet (MSDS), Certificate of Calibration and Traceability to NIST
    Storage Requirements : Store at room temperature or keep refrigerated. Keep bottle tightly sealed to avoid contamination.
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